Review



napi iib  (ABclonal Biotechnology)


Bioz Verified Symbol ABclonal Biotechnology is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ABclonal Biotechnology napi iib
    Napi Iib, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/napi+iib/SLC34A2+Rabbit+pAb/pm42705192-87-13-17
    Average 94 stars, based on 5 article reviews
    napi iib - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Membrane:

    Article Title: MAPK signaling pathway participates in the regulation of intestinal phosphorus and calcium absorption in broiler chickens via 1,25-dihydroxyvitamin D 3 .
    Article Snippet: .. The PVDF membrane was then blocked with 5% nonfat powdered milk in Tris-buffered saline supplemented with tween-20 (TBST) at 37°C for 1 h. Subsequently, the membranes were incubated overnight at 4°C with primary antibodies against GAPDH (1:10,000, 60004-1- Ig, Proteintech, Wuhan, China), NaPi-IIb (1:1,000, A9460, ABclonal, Wuhan, China), CaBP-D28k (1:5,000, 14479-1-AP, Proteintech, Wuhan, China), p-ERK (1:1,000, 9101S, Cell Signaling Technology, Boston, USA), t-ERK (1:8,000, 11257-1-AP-50, Proteintech, Wuhan, China), p-p38MAPK (1:1,000, 9216S, Cell Signaling Technology, Boston, USA), and t-p38MAPK (1:1,000, 14064-1-AP, Proteintech, Wuhan, China). ..

    Saline:

    Article Title: MAPK signaling pathway participates in the regulation of intestinal phosphorus and calcium absorption in broiler chickens via 1,25-dihydroxyvitamin D 3 .
    Article Snippet: .. The PVDF membrane was then blocked with 5% nonfat powdered milk in Tris-buffered saline supplemented with tween-20 (TBST) at 37°C for 1 h. Subsequently, the membranes were incubated overnight at 4°C with primary antibodies against GAPDH (1:10,000, 60004-1- Ig, Proteintech, Wuhan, China), NaPi-IIb (1:1,000, A9460, ABclonal, Wuhan, China), CaBP-D28k (1:5,000, 14479-1-AP, Proteintech, Wuhan, China), p-ERK (1:1,000, 9101S, Cell Signaling Technology, Boston, USA), t-ERK (1:8,000, 11257-1-AP-50, Proteintech, Wuhan, China), p-p38MAPK (1:1,000, 9216S, Cell Signaling Technology, Boston, USA), and t-p38MAPK (1:1,000, 14064-1-AP, Proteintech, Wuhan, China). ..

    Incubation:

    Article Title: MAPK signaling pathway participates in the regulation of intestinal phosphorus and calcium absorption in broiler chickens via 1,25-dihydroxyvitamin D 3 .
    Article Snippet: .. The PVDF membrane was then blocked with 5% nonfat powdered milk in Tris-buffered saline supplemented with tween-20 (TBST) at 37°C for 1 h. Subsequently, the membranes were incubated overnight at 4°C with primary antibodies against GAPDH (1:10,000, 60004-1- Ig, Proteintech, Wuhan, China), NaPi-IIb (1:1,000, A9460, ABclonal, Wuhan, China), CaBP-D28k (1:5,000, 14479-1-AP, Proteintech, Wuhan, China), p-ERK (1:1,000, 9101S, Cell Signaling Technology, Boston, USA), t-ERK (1:8,000, 11257-1-AP-50, Proteintech, Wuhan, China), p-p38MAPK (1:1,000, 9216S, Cell Signaling Technology, Boston, USA), and t-p38MAPK (1:1,000, 14064-1-AP, Proteintech, Wuhan, China). ..

    Article Title: 1,25-dihydroxyvitamin D 3 enhances the expression of phosphorus transporters via vitamin D receptor in ligated duodenal loops of Arbor Acres male broilers.
    Article Snippet: .. The membranes were washed with TBST, and then incubated with primary antibodies against VDR (Cat. No. ab109234, Abcam, 1:5,000), NaPi-IIb (Cat. No. A9460, ABclonal, 1:1, 000), PiT-1 (Cat. No. A4117, ABclonal, 1:1,000), PiT-2 (Cat. No. A6739, ABclonal, 1:1,000), and β-actin (Cat. No. HX1829, Huaxingbio, 1:5,000) at 4◦C overnight, followed by incubation with a Goat Anti-Rabbit IgG Secondary Antibody (HRP) (Cat. No. HX2031, Huaxingbio, 1:5,000). .. Images were recorded using a Tanon Chemiluminescence Image Scanner, and the band intensity was analyzed using the Tanon GIS software (Tanon, Shanghai, China).

    Article Title: Vitamin D receptor silencing impairs the 1,25-dihydroxyvitamin D 3 -stimulated phosphorus absorption by suppressing NaPi-IIb gene expression in primary duodenal epithelial cells of broilers.
    Article Snippet: Total protein (20 μg) was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto a PVDF membrane (catalog number IPVH00001; Merck Millipore, USA) and blocked with 5% (w/v) bovine serum albumin. .. Membranes were then incubated overnight with VDR (catalog number ab109234; abcam, UK; 1:5,000), NaPi-IIb (catalog number A9460; ABclonal, China; 1:1,000) or β-Tubulin (catalog number HX1847; Huaxingbio, China; 1:10,000) primary antibodies, and the next day with goat anti-rabbit (catalog number HX2031; Huaxingbio, China; 1:10,000) or goat anti-mouse (catalog number HX2032; Huaxingbio, China; 1:10,000) secondary antibodies. .. The protein bands were visualized using Tanon Chemiluminescence Image Scanner (5200, Tanon, Shanghai, China) and quantified using its own Gis software.



    Similar Products

    94
    ABclonal Biotechnology napi iib
    Napi Iib, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/napi+iib/SLC34A2+Rabbit+pAb/pm42705192-87-13-17
    Average 94 stars, based on 1 article reviews
    napi iib - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    94
    ABclonal Biotechnology antibodies against napi iib
    Effects of dietary vitamin D 3 supplementation on <t>jejunal</t> <t>NaPi-IIb</t> mRNA and protein expression in broiler chickens (19 d) (Experiment 1). The control and vitamin D 3 diets contained 0 and 1000 IU/kg vitamin D 3 , respectively. (a) NaPi-IIb mRNA expression. (b, c) Representative immunoblots and quantitative analysis of NaPi-IIb protein expression. NaPi-IIb mRNA expression values are means ± SD of six replicates per treatment (1 broiler per replicate) (n = 6). NaPi-IIb protein abundance values are means ± SD of four replicates per treatment (1 broiler per replicate) (n = 4). Values with different letters differ between treatments ( P < 0.05).
    Antibodies Against Napi Iib, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/napi+iib/SLC34A2+Rabbit+pAb/pmc13011214-154-6-11
    Average 94 stars, based on 1 article reviews
    antibodies against napi iib - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    90
    Genotech Corp primers for p transporters ( pit-1, pit-2 , and napi-iib)
    Effects of dietary vitamin D 3 supplementation on <t>jejunal</t> <t>NaPi-IIb</t> mRNA and protein expression in broiler chickens (19 d) (Experiment 1). The control and vitamin D 3 diets contained 0 and 1000 IU/kg vitamin D 3 , respectively. (a) NaPi-IIb mRNA expression. (b, c) Representative immunoblots and quantitative analysis of NaPi-IIb protein expression. NaPi-IIb mRNA expression values are means ± SD of six replicates per treatment (1 broiler per replicate) (n = 6). NaPi-IIb protein abundance values are means ± SD of four replicates per treatment (1 broiler per replicate) (n = 4). Values with different letters differ between treatments ( P < 0.05).
    Primers For P Transporters ( Pit 1, Pit 2 , And Napi Iib), supplied by Genotech Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/napi+iib/primers+for+p+transporters+++pit+1++pit+2+++and+napi+iib+/pmc11564020-163-43-66
    Average 90 stars, based on 1 article reviews
    primers for p transporters ( pit-1, pit-2 , and napi-iib) - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Charles River Laboratories rats with constitutive depletion of napi-iib/ slc34a2
    ( A ) Genotype and gender from pups from 9 litters born from two independent heterozygous <t>Slc34a2</t> breedings. ( B ) body weight, ( C ) food intake, ( D ) water intake, ( E ) fecal output and ( F ) urinary output of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM.
    Rats With Constitutive Depletion Of Napi Iib/ Slc34a2, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/napi+iib/rats+with+constitutive+depletion+of+napi+iib++slc34a2/pmc08042035-136-5-10
    Average 90 stars, based on 1 article reviews
    rats with constitutive depletion of napi-iib/ slc34a2 - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    91
    Proteintech napi iib
    ( A ) Genotype and gender from pups from 9 litters born from two independent heterozygous <t>Slc34a2</t> breedings. ( B ) body weight, ( C ) food intake, ( D ) water intake, ( E ) fecal output and ( F ) urinary output of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM.
    Napi Iib, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/napi+iib/SLC34A2+Antibody/10__1113_slash_jp280345-122-27-44
    Average 91 stars, based on 1 article reviews
    napi iib - by Bioz Stars, 2026-10
    91/100 stars
      Buy from Supplier

    90
    Biomol GmbH napi-iib (slc34a1; #vmps-6022)
    ( A ) Genotype and gender from pups from 9 litters born from two independent heterozygous <t>Slc34a2</t> breedings. ( B ) body weight, ( C ) food intake, ( D ) water intake, ( E ) fecal output and ( F ) urinary output of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM.
    Napi Iib (Slc34a1; #Vmps 6022), supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/napi+iib/napi+iib++slc34a1+++vmps+6022+/pm30715488-87-2-4
    Average 90 stars, based on 1 article reviews
    napi-iib (slc34a1; #vmps-6022) - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Global Napi Pharmaceuticals Company S A E global napi-iib knockout
    ( A ) Genotype and gender from pups from 9 litters born from two independent heterozygous <t>Slc34a2</t> breedings. ( B ) body weight, ( C ) food intake, ( D ) water intake, ( E ) fecal output and ( F ) urinary output of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM.
    Global Napi Iib Knockout, supplied by Global Napi Pharmaceuticals Company S A E, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/napi+iib/global+napi+iib+knockout/10__5603_slash_ep__a2019__0050-82-0-0
    Average 90 stars, based on 1 article reviews
    global napi-iib knockout - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Microsynth ag primers and probes for mouse fgf23, hepcidin, napi-iib, vdr and cyp27b1
    ( A ) Genotype and gender from pups from 9 litters born from two independent heterozygous <t>Slc34a2</t> breedings. ( B ) body weight, ( C ) food intake, ( D ) water intake, ( E ) fecal output and ( F ) urinary output of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM.
    Primers And Probes For Mouse Fgf23, Hepcidin, Napi Iib, Vdr And Cyp27b1, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/napi+iib/primers+for+gapdh++bmp2++ccdn1++cd90++colia1++dspp++oct4++dmp+1++and+runx2/pmc06802194-59-20-34
    Average 90 stars, based on 1 article reviews
    primers and probes for mouse fgf23, hepcidin, napi-iib, vdr and cyp27b1 - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results


    Effects of dietary vitamin D 3 supplementation on jejunal NaPi-IIb mRNA and protein expression in broiler chickens (19 d) (Experiment 1). The control and vitamin D 3 diets contained 0 and 1000 IU/kg vitamin D 3 , respectively. (a) NaPi-IIb mRNA expression. (b, c) Representative immunoblots and quantitative analysis of NaPi-IIb protein expression. NaPi-IIb mRNA expression values are means ± SD of six replicates per treatment (1 broiler per replicate) (n = 6). NaPi-IIb protein abundance values are means ± SD of four replicates per treatment (1 broiler per replicate) (n = 4). Values with different letters differ between treatments ( P < 0.05).

    Journal: Poultry Science

    Article Title: PKA and PKC signaling pathways mediate vitamin D₃-regulated intestinal phosphorus absorption in broiler chickens

    doi: 10.1016/j.psj.2026.106762

    Figure Lengend Snippet: Effects of dietary vitamin D 3 supplementation on jejunal NaPi-IIb mRNA and protein expression in broiler chickens (19 d) (Experiment 1). The control and vitamin D 3 diets contained 0 and 1000 IU/kg vitamin D 3 , respectively. (a) NaPi-IIb mRNA expression. (b, c) Representative immunoblots and quantitative analysis of NaPi-IIb protein expression. NaPi-IIb mRNA expression values are means ± SD of six replicates per treatment (1 broiler per replicate) (n = 6). NaPi-IIb protein abundance values are means ± SD of four replicates per treatment (1 broiler per replicate) (n = 4). Values with different letters differ between treatments ( P < 0.05).

    Article Snippet: PVDF membranes were incubated with primary antibodies against NaPi-IIb (1:1000, A9460; ABclonal, Wuhan, China), phosphorylated PKA (p-PKA; 1:1000, 5661S), phosphorylated PI3K (p-PI3K; 1:1000, 4228), phosphorylated ERK (p-ERK; 1:1000, 9101S), phosphorylated p38MAPK (p-p38MAPK; 1:1000, 9216S), total PKA (t-PKA; 1:1000, 5842S) (Cell Signaling Technology, Boston, USA), total PI3K (t-PI3K; 1:10000, 60225-1-Ig), total ERK (t-ERK; 1:8000, 11257-1-AP-50), total p38MAPK(t-p38MAPK; 1:1000, 14064-1-AP), total PKC (t-PKC; 1:1000, 21991-1-AP), phosphorylated PKC (p-PKC; 1:5000, 29123-1-AP), and GAPDH (1:10000, 60004-1-Ig) (Proteintech, Wuhan, China).

    Techniques: Expressing, Control, Western Blot, Quantitative Proteomics

    Effects of the PKA inhibitor H-89 on jejunal NaPi-IIb mRNA and protein expression in broiler chickens (10–15 d) (Experiment 2). (a) NaPi-IIb mRNA expression. (b, c) Representative immunoblots and quantitative analysis of NaPi-IIb protein expression. NaPi-IIb mRNA expression values are means ± SD of six replicates per treatment (1 broiler per replicate) (n = 6). NaPi-IIb protein abundance values are means ± SD of four replicates per treatment (1 broiler per replicate) (n = 4). Values with different letters differ between treatments ( P < 0.05).

    Journal: Poultry Science

    Article Title: PKA and PKC signaling pathways mediate vitamin D₃-regulated intestinal phosphorus absorption in broiler chickens

    doi: 10.1016/j.psj.2026.106762

    Figure Lengend Snippet: Effects of the PKA inhibitor H-89 on jejunal NaPi-IIb mRNA and protein expression in broiler chickens (10–15 d) (Experiment 2). (a) NaPi-IIb mRNA expression. (b, c) Representative immunoblots and quantitative analysis of NaPi-IIb protein expression. NaPi-IIb mRNA expression values are means ± SD of six replicates per treatment (1 broiler per replicate) (n = 6). NaPi-IIb protein abundance values are means ± SD of four replicates per treatment (1 broiler per replicate) (n = 4). Values with different letters differ between treatments ( P < 0.05).

    Article Snippet: PVDF membranes were incubated with primary antibodies against NaPi-IIb (1:1000, A9460; ABclonal, Wuhan, China), phosphorylated PKA (p-PKA; 1:1000, 5661S), phosphorylated PI3K (p-PI3K; 1:1000, 4228), phosphorylated ERK (p-ERK; 1:1000, 9101S), phosphorylated p38MAPK (p-p38MAPK; 1:1000, 9216S), total PKA (t-PKA; 1:1000, 5842S) (Cell Signaling Technology, Boston, USA), total PI3K (t-PI3K; 1:10000, 60225-1-Ig), total ERK (t-ERK; 1:8000, 11257-1-AP-50), total p38MAPK(t-p38MAPK; 1:1000, 14064-1-AP), total PKC (t-PKC; 1:1000, 21991-1-AP), phosphorylated PKC (p-PKC; 1:5000, 29123-1-AP), and GAPDH (1:10000, 60004-1-Ig) (Proteintech, Wuhan, China).

    Techniques: Expressing, Western Blot, Quantitative Proteomics

    Effects of the PKC inhibitor staurosporine on duodenal NaPi-IIb mRNA and protein expression in broiler chickens (9–13 d) (Experiment 3). (a) NaPi-IIb mRNA expression. (b, c) Representative immunoblots and quantitative analysis of NaPi-IIb protein expression. NaPi-IIb mRNA expression values are means ± SD of six replicates per treatment (1 broiler per replicate) (n = 6.) NaPi-IIb protein abundance values are means ± SD of four replicates per treatment (1 broiler per replicate) (n = 4). Values with different letters differ between treatments ( P < 0.05).

    Journal: Poultry Science

    Article Title: PKA and PKC signaling pathways mediate vitamin D₃-regulated intestinal phosphorus absorption in broiler chickens

    doi: 10.1016/j.psj.2026.106762

    Figure Lengend Snippet: Effects of the PKC inhibitor staurosporine on duodenal NaPi-IIb mRNA and protein expression in broiler chickens (9–13 d) (Experiment 3). (a) NaPi-IIb mRNA expression. (b, c) Representative immunoblots and quantitative analysis of NaPi-IIb protein expression. NaPi-IIb mRNA expression values are means ± SD of six replicates per treatment (1 broiler per replicate) (n = 6.) NaPi-IIb protein abundance values are means ± SD of four replicates per treatment (1 broiler per replicate) (n = 4). Values with different letters differ between treatments ( P < 0.05).

    Article Snippet: PVDF membranes were incubated with primary antibodies against NaPi-IIb (1:1000, A9460; ABclonal, Wuhan, China), phosphorylated PKA (p-PKA; 1:1000, 5661S), phosphorylated PI3K (p-PI3K; 1:1000, 4228), phosphorylated ERK (p-ERK; 1:1000, 9101S), phosphorylated p38MAPK (p-p38MAPK; 1:1000, 9216S), total PKA (t-PKA; 1:1000, 5842S) (Cell Signaling Technology, Boston, USA), total PI3K (t-PI3K; 1:10000, 60225-1-Ig), total ERK (t-ERK; 1:8000, 11257-1-AP-50), total p38MAPK(t-p38MAPK; 1:1000, 14064-1-AP), total PKC (t-PKC; 1:1000, 21991-1-AP), phosphorylated PKC (p-PKC; 1:5000, 29123-1-AP), and GAPDH (1:10000, 60004-1-Ig) (Proteintech, Wuhan, China).

    Techniques: Expressing, Western Blot, Quantitative Proteomics

    ( A ) Genotype and gender from pups from 9 litters born from two independent heterozygous Slc34a2 breedings. ( B ) body weight, ( C ) food intake, ( D ) water intake, ( E ) fecal output and ( F ) urinary output of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM.

    Journal: Scientific Reports

    Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

    doi: 10.1038/s41598-021-86874-z

    Figure Lengend Snippet: ( A ) Genotype and gender from pups from 9 litters born from two independent heterozygous Slc34a2 breedings. ( B ) body weight, ( C ) food intake, ( D ) water intake, ( E ) fecal output and ( F ) urinary output of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM.

    Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

    Techniques:

    ( A ) Fecal Pi, ( B ) fecal Ca 2+ , ( C ) urinary Pi, ( D ) urinary Ca 2+ and ( E ) urinary creatinine of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM. Statistical significance was calculated by ANOVA-Bonferroni. * P ≤ 0.05 and **** P ≤ 0.0001.

    Journal: Scientific Reports

    Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

    doi: 10.1038/s41598-021-86874-z

    Figure Lengend Snippet: ( A ) Fecal Pi, ( B ) fecal Ca 2+ , ( C ) urinary Pi, ( D ) urinary Ca 2+ and ( E ) urinary creatinine of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM. Statistical significance was calculated by ANOVA-Bonferroni. * P ≤ 0.05 and **** P ≤ 0.0001.

    Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

    Techniques:

    ( A ) Plasma Pi, ( B ) plasma Ca 2+ , ( C ) plasma intact FGF-23 and ( D ) plasma 1,25(OH) 2 vitamin D 3 of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM. Statistical significance was calculated by ANOVA-Bonferroni. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

    Journal: Scientific Reports

    Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

    doi: 10.1038/s41598-021-86874-z

    Figure Lengend Snippet: ( A ) Plasma Pi, ( B ) plasma Ca 2+ , ( C ) plasma intact FGF-23 and ( D ) plasma 1,25(OH) 2 vitamin D 3 of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM. Statistical significance was calculated by ANOVA-Bonferroni. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

    Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

    Techniques: Clinical Proteomics

    Expression of NaPi-IIb in total membranes isolated from mucosa of ( A ) duodenum and ( B ) jejunum from wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats challenged for 3 days with low Pi diet. The abundance of the cotransporter was normalize to the expression of actin. The ratio in WT rats was considered as 100%.

    Journal: Scientific Reports

    Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

    doi: 10.1038/s41598-021-86874-z

    Figure Lengend Snippet: Expression of NaPi-IIb in total membranes isolated from mucosa of ( A ) duodenum and ( B ) jejunum from wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats challenged for 3 days with low Pi diet. The abundance of the cotransporter was normalize to the expression of actin. The ratio in WT rats was considered as 100%.

    Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

    Techniques: Expressing, Isolation

    Intestines from ( A , C ) wild type and ( B , D ) Slc34a2 homozygous E18 embryos stained with ( A , B ) H&E or with ( C , D ) a NaPi-IIb antibody. Scale bars: 100 µm in ( A , B ); 50 µm in ( C , D ).

    Journal: Scientific Reports

    Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

    doi: 10.1038/s41598-021-86874-z

    Figure Lengend Snippet: Intestines from ( A , C ) wild type and ( B , D ) Slc34a2 homozygous E18 embryos stained with ( A , B ) H&E or with ( C , D ) a NaPi-IIb antibody. Scale bars: 100 µm in ( A , B ); 50 µm in ( C , D ).

    Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

    Techniques: Staining

    Lungs from ( A , C ) wild type and ( B , D ) Slc34a2 homozygous E18 embryos stained with ( A , B ) H&E or with ( C , D ) a NaPi-IIb antibody. Scale bars: 100 µm in ( A , B ); 50 µm in ( C , D ).

    Journal: Scientific Reports

    Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

    doi: 10.1038/s41598-021-86874-z

    Figure Lengend Snippet: Lungs from ( A , C ) wild type and ( B , D ) Slc34a2 homozygous E18 embryos stained with ( A , B ) H&E or with ( C , D ) a NaPi-IIb antibody. Scale bars: 100 µm in ( A , B ); 50 µm in ( C , D ).

    Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

    Techniques: Staining

    Liver ( A , B ) and pancreas ( C , D ) from wild type ( A , C ) and Slc34a2 homozygous E18 embryos ( B , D ) stained with a NaPi-IIb antibody. Scale bars: 50 µm.

    Journal: Scientific Reports

    Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

    doi: 10.1038/s41598-021-86874-z

    Figure Lengend Snippet: Liver ( A , B ) and pancreas ( C , D ) from wild type ( A , C ) and Slc34a2 homozygous E18 embryos ( B , D ) stained with a NaPi-IIb antibody. Scale bars: 50 µm.

    Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

    Techniques: Staining

    Kidneys from ( A ) wild type and ( B ) Slc34a2 homozygous E18 embryos stained with H&E. Scale bars: 250 µm.

    Journal: Scientific Reports

    Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

    doi: 10.1038/s41598-021-86874-z

    Figure Lengend Snippet: Kidneys from ( A ) wild type and ( B ) Slc34a2 homozygous E18 embryos stained with H&E. Scale bars: 250 µm.

    Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

    Techniques: Staining